ARG83428

Indoleacetic Acid Oxidase Assay Kit

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96 wells

459.00

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Datasheet
Protocols

Overview

Product Description	ARG83428 Indoleacetic Acid Oxidase Assay Kit can be used to measure Indoleacetic Acid Oxidase in tissue extracts, cell lysate, cell culture media and other biological fluids.
Tested Reactivity	Other
Tested Application	FuncSt
Target Name	Indoleacetic Acid Oxidase
Conjugation Note	Read at 530 nm
Sensitivity	0.1 μmol/ml
Sample Type	Tissue extracts, cell lysate, cell culture media and other biological fluids.
Standard Range	0.1 μmol/ml - 2 μmol/ml
Sample Volume	10 μl

Application Instructions

Assay Time	1 hr

Properties

Form	96 well
Storage Instruction	Store the kit at 4°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background	Indoleacetic Acid Oxidase (IAAO), an enzyme that oxidizes and breaks down indoleacetic acid in plants. An iron-containing hemoglobin, manganese and monohydric phenols are required as cofactors. The final products of oxidation are physiologically inactive 3-methyleneoxindole and 3-methyloxindole. The shoot and root tips contain less IAA oxidase than older tissues. The further away from the shoot or root tip, the higher the enzyme activity. In dwarf plants, the activity of IAA oxidase is high, which restricts the growth of plants and shows the characteristics of dwarf.

Background

Indoleacetic Acid Oxidase (IAAO), an enzyme that oxidizes and breaks down indoleacetic acid in plants. An iron-containing hemoglobin, manganese and monohydric phenols are required as cofactors. The final products of oxidation are physiologically inactive 3-methyleneoxindole and 3-methyloxindole. The shoot and root tips contain less IAA oxidase than older tissues. The further away from the shoot or root tip, the higher the enzyme activity. In dwarf plants, the activity of IAA oxidase is high, which restricts the growth of plants and shows the characteristics of dwarf.