ARG82320
Hydroxyproline (Total Collagen) Assay Kit
Hydroxyproline (Total Collagen) Assay Kit for Functional study and Human,Mouse,Rat,Mammal
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82320-001 | 96-well plate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82320-002 | Collagen standard (1200 µg/ml) | 1 vial | 4°C |
| ARG82320-003 | Assay buffer | 1 vial | 4°C |
| ARG82320-004 | Detection reagent A | 2 vials | 4°C |
| ARG82320-005 | Detection reagent B | 1 vial | 4°C |
| ARG82320-006 | Special screw-capped tubes | 100 ea | Room temperature |
| ARG82320-007 | Plate sealer | 2 strips | Room temperature |
Overview
| Product Description | ARG82320 Hydroxyproline (Total Collagen) Assay Kit is a detection kit for the quantification of Hydroxyproline in tissue and cell extracts which can be used as a direct measure of the amount of collagen present. |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mamm |
| Tested Application | FuncSt |
| Specificity | The kit is based on the detection of hydroxyproline. Hydroxyproline is a non-proteinogenic amino acid, which in mammals occurs in elastin and collagen. Its presence is mainly limited to the triple helix of collagen, where its presence increases the triple helix stability. Hydroxyproline is formed posttranslationally from specific proline residues by action of the enzyme prolylhydroxylase. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present. |
| Target Name | Hydroxyproline (Total Collagen) |
| Conjugation Note | Read at 570 nm. |
| Sensitivity | 2.5 µg/ml |
| Sample Type | Tissue and cell extracts. |
| Standard Range | 6.25 - 300 µg/ml |
| Sample Volume | Tissue: 100 µl Cell extracts: 50 µl |
| Alternate Names | OI1; OI2; OI3; OI4; EDSC; Collagen alpha-1(I) chain; Alpha-1 type I collagen |
Application Instructions
| Assay Time | ~ 1.5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | COL1A1 |
| Gene Full Name | collagen, type I, alpha 1 |
| Background | This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008] |
| Function | Type I collagen is a member of group I collagen (fibrillar forming collagen). [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. [UniProt] |
| PTM | Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82320 Hydroxyproline (Total Collagen) Assay Kit standard curve image
ARG82320 Hydroxyproline (Total Collagen) Assay Kit results of a typical standard run with optical density reading at 570 nm.
| Title | Download Link |
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| ARG82320 Hydroxyproline (Total Collagen) Assay Kit User manual |
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