ARG81311

Hydroxyproline Assay Kit

Hydroxyproline Assay Kit for Functional study and Human,Mammal,Mouse,Rat

Component

Cat No Component Name Package
ARG81311-1 Standard 100 µl
ARG81311-2 Assay Buffer 12 ml
ARG81311-3 Chloramine T Reagent 600 µl
ARG81311-4 Ehrlich’s reagent concentrate (2X) 5 ml
ARG81311-5 Ehrlich’s Diluent 5 ml

Overview

Product Description ARG81311 Hydroxyproline Assay Kit is a detection kit for the quantification of Hydroxyproline in serum, plasma, urine and cell / tissue lysates.
Tested Reactivity Hu, Ms, Rat, Mamm
Tested Application FuncSt
Target Name Hydroxyproline
Conjugation Note Read at 540 - 560 nm.
Sensitivity 6.25 µg/ml
Sample Type Serum, plasma, urine and cell / tissue lysates.
Standard Range 6.25 - 100 µg/ml
Sample Volume 10 µl
Alternate Names L-hydroxyproline; Hyp; 4-Hydroxyproline

Application Instructions

Assay Time ~ 3 hours

Properties

Form Liquid
Storage Instruction Store the kit at 2-8°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background Hydroxyproline is produced by hydroxylation of the amino acid proline by the enzyme prolyl hydroxylase following protein synthesis (as a post-translational modification). The enzyme catalyzed reaction takes place in the lumen of the endoplasmic reticulum. [Wikipedia]

Images (2) Click the Picture to Zoom In

  • ARG81311 Hydroxyproline Assay Kit standard curve image

    ARG81311 Hydroxyproline Assay Kit results of a typical standard run with optical density reading at 540 nm.

  • ARG81311 Hydroxyproline Assay Kit sample detection image

    Hydrolyzed serum, untreated serum and water samples tested with ARG81311 Hydroxyproline Assay Kit (OD reading at 540 nm).

Title Download Link
ARG81311 Hydroxyproline Assay Kit User's manual download icon Download