ARG81311
Hydroxyproline Assay Kit
Hydroxyproline Assay Kit for Functional study and Human,Mammal,Mouse,Rat
Component
Cat No | Component Name | Package |
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ARG81311-1 | Standard | 100 µl |
ARG81311-2 | Assay Buffer | 12 ml |
ARG81311-3 | Chloramine T Reagent | 600 µl |
ARG81311-4 | Ehrlich’s reagent concentrate (2X) | 5 ml |
ARG81311-5 | Ehrlich’s Diluent | 5 ml |
Overview
Product Description | ARG81311 Hydroxyproline Assay Kit is a detection kit for the quantification of Hydroxyproline in serum, plasma, urine and cell / tissue lysates. |
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Tested Reactivity | Hu, Ms, Rat, Mamm |
Tested Application | FuncSt |
Target Name | Hydroxyproline |
Conjugation Note | Read at 540 - 560 nm. |
Sensitivity | 6.25 µg/ml |
Sample Type | Serum, plasma, urine and cell / tissue lysates. |
Standard Range | 6.25 - 100 µg/ml |
Sample Volume | 10 µl |
Alternate Names | L-hydroxyproline; Hyp; 4-Hydroxyproline |
Application Instructions
Assay Time | ~ 3 hours |
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Properties
Form | Liquid |
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Storage Instruction | Store the kit at 2-8°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Background | Hydroxyproline is produced by hydroxylation of the amino acid proline by the enzyme prolyl hydroxylase following protein synthesis (as a post-translational modification). The enzyme catalyzed reaction takes place in the lumen of the endoplasmic reticulum. [Wikipedia] |
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Images (2) Click the Picture to Zoom In
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ARG81311 Hydroxyproline Assay Kit standard curve image
ARG81311 Hydroxyproline Assay Kit results of a typical standard run with optical density reading at 540 nm.
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ARG81311 Hydroxyproline Assay Kit sample detection image
Hydrolyzed serum, untreated serum and water samples tested with ARG81311 Hydroxyproline Assay Kit (OD reading at 540 nm).
Title | Download Link |
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ARG81311 Hydroxyproline Assay Kit User's manual | Download |