ARG81423
Human uPAR ELISA Kit
Human uPAR ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81423-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81423-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81423-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81423-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81423-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81423-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81423-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81423-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81423-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81423-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81423-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81423 Human uPAR ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human uPAR in serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | uPAR |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.4% Inter-Assay CV: 7.5% |
| Alternate Names | Monocyte activation antigen Mo3; CD antigen CD87; uPAR; U-PAR; URKR; Urokinase plasminogen activator surface receptor; UPAR; CD87 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q03405 Human Urokinase plasminogen activator surface receptor |
|---|---|
| Gene Symbol | PLAUR |
| Gene Full Name | plasminogen activator, urokinase receptor |
| Background | This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq, Jul 2008] |
| Function | Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. [UniProt] |
| Highlight | Related products: uPAR antibodies; uPAR ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81423 Human uPAR ELISA Kit standard curve image
ARG81423 Human uPAR ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81423 Human uPAR ELISA Kit User's manual |
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