ARG80183
Human sRANK Ligand / TNFSF11 / TRANCE ELISA Kit
Human sRANK Ligand / TNFSF11 / TRANCE ELISA Kit for ELISA and Human
Cell Biology and Cellular Response kit; Immune System kit
Publication1
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80183-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80183-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80183-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80183-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80183-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80183-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80183-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80183-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80183-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80183-010 | STOP solution | 12ml | 4°C |
| ARG80183-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80183 Human sRANK Ligand / TNFSF11 / TRANCE ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human sRANK Ligand / TNFSF11 / TRANCE in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human IL-2,3,4,5,6,7,9,10,12, IFN- gamma, TNF- alpha |
| Target Name | sRANK Ligand / TNFSF11 / TRANCE |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 8 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | TRANCE; Osteoprotegerin ligand; CD254; sOdf; Receptor activator of nuclear factor kappa-B ligand; OPTB2; RANKL; OPGL; Tumor necrosis factor ligand superfamily member 11; hRANKL2; TNF-related activation-induced cytokine; Osteoclast differentiation factor; ODF; CD antigen CD254 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O14788 Human Tumor necrosis factor ligand superfamily member 11 |
|---|---|
| Gene Symbol | TNFSF11 |
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 11 |
| Background | This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced tra |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit; Immune System kit |
| PTM | The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. |
Images (1) Click the Picture to Zoom In
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ARG80183 Human sRANK Ligand / TNFSF11 / TRANCE ELISA Kit standard curve image
ARG80183 Human sRANK Ligand / TNFSF11 / TRANCE ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
sTNFRII-Fc modification protects human UC-MSCs against apoptosis/autophagy induced by TNF-α and enhances their efficacy in alleviating inflammatory arthritis.
ELISA / Human / Cell culture medium
| Title | Download Link |
|---|---|
| ARG80183 Human sRANK Ligand / TNFSF11 / TRANCE ELISA Kit User's manual |
 Download
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