ARG81527
Human sCD6 ELISA Kit
Human sCD6 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81527-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81527-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81527-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81527-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81527-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81527-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81527-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81527-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81527-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81527-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81527-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81527 Human sCD6 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human sCD6 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | sCD6 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2% Inter-Assay CV: 6.7% |
| Alternate Names | CD antigen CD6; TP120; T-cell differentiation antigen CD6; T12 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P30203 Human T-cell differentiation antigen CD6 |
|---|---|
| Gene Symbol | CD6 |
| Gene Full Name | CD6 molecule |
| Background | This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. This gene may be associated with susceptibility to multiple sclerosis (PMID: 19525953, 21849685). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| Function | Involved in cell adhesion. Binds to CD166. [UniProt] |
| Highlight | Related products: CD6 antibodies; CD6 ELISA Kits; CD6 Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues. Glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81527 Human sCD6 ELISA Kit standard curve image
ARG81527 Human sCD6 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81527 Human sCD6 ELISA Kit User's manual |
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