ARG80133
Human beta NGF ELISA Kit
Human beta NGF ELISA Kit for ELISA and Human
Neuroscience kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80133-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80133-002 | Standard (Lyophilized) | 3 X 1.2 ng/vial | 4°C |
| ARG80133-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80133-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80133-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80133-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80133-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80133-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80133-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80133-010 | STOP solution | 12ml | 4°C |
| ARG80133-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80133 Human beta NGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human beta NGF in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human Artemin, BDNF, CNTF, EG-VEGF, EGF, NT-3, NT-4, OPG, FGF-9, Galectin-1, GDNF, Midkine, Neurturin, Persephin, Pleiotrophin, RELM- beta, Resistin, VEGF；rat CNTF |
| Target Name | beta NGF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 4 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 7.8 - 500 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | HSAN5; Beta-nerve growth factor; Beta-NGF; NGFB |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NGF |
| Gene Full Name | nerve growth factor (beta polypeptide) |
| Background | This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008] |
| Function | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). [UniProt] |
| Highlight | Related products: beta NGF antibodies; beta NGF ELISA Kits; beta NGF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Neuroscience kit |
Images (1) Click the Picture to Zoom In
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ARG80133 Human beta NGF ELISA Kit standard curve image
ARG80133 Human beta NGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80133 Human beta NGF ELISA Kit User's manual |
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