ARG81612
Human alpha 1 microglobulin ELISA Kit
Human alpha 1 microglobulin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81612-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81612-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81612-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81612-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81612-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81612-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81612-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81612-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81612-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81612-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81612-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81612 Human alpha 1 microglobulin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human alpha 1 microglobulin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | alpha 1 microglobulin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3% Inter-Assay CV: 6.7% |
| Alternate Names | Bikunin; HCP; ITIL; ITILC; ITI; ITI-LC; UTI; Protein HC; Complex-forming glycoprotein heterogeneous in charge; EDC1; HI30; HI-30; Alpha-1 microglycoprotein; A1M; IATIL; Uronic-acid-rich protein; Protein AMBP |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AMBP |
| Gene Full Name | alpha-1-microglobulin/bikunin precursor |
| Background | This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008] |
| Function | Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization. Trypstatin is a trypsin inhibitor. [UniProt] |
| Highlight | Related products: alpha 1 microglobulin antibodies; alpha 1 microglobulin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The precursor is proteolytically processed into separately functioning proteins. 3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'. Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate. N- and O-glycosylated. N-glycan heterogeneity at Asn-115: Hex5HexNAc4 (major), Hex6HexNAc5 (minor) and dHex1Hex6HexNAc5 (minor). N-glycan at Asn-250: Hex5HexNAc4. O-linkage of the glycosaminoglycan, chondroitin sulfate, at Ser-215 allows cross-linking between the three polypeptide chains. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81612 Human alpha 1 microglobulin ELISA Kit standard curve image
ARG81612 Human alpha 1 microglobulin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81612 Human alpha 1 microglobulin ELISA Kit User's manual |
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