ARG81598
Human WIF1 ELISA Kit
Human WIF1 ELISA Kit for ELISA and Human
Publication1
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81598-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81598-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81598-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81598-004 | Antibody conjugate concentrate (100X) | 1 vial (130 µl) | 4°C |
| ARG81598-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81598-006 | HRP-Streptavidin concentrate (100X) | 1 vial (130 µl) | 4°C |
| ARG81598-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81598-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81598-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81598-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81598-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81598 Human WIF1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human WIF1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | WIF1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 11.7 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 23.4 - 1500 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.1% Inter-Assay CV: 6.9% |
| Alternate Names | WIF-1; Wnt inhibitory factor 1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | WIF1 |
| Gene Full Name | WNT inhibitory factor 1 |
| Background | The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. [provided by RefSeq, Jun 2010] |
| Function | Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation. [UniProt] |
| Highlight | Related products: WIF1 antibodies; WIF1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81598 Human WIF1 ELISA Kit standard curve image
ARG81598 Human WIF1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
Epigenetic regulation of human WIF1 and DNA methylation situation of WIF1 and GSTM5 in urothelial carcinoma
ELISA / Human/Cell culture supernatants
| Title | Download Link |
|---|---|
| ARG81598 Human WIF1 ELISA Kit user's manual |
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