ARG82584
Human VEGFD ELISA Kit
Human VEGFD ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82584 Human VEGFD ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human VEGFD in serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | This kit could assay both natural and recombinant Human VEGFD. No significant cross-reactivity or interference was observed in the following samples: Human: beta NGF, EGF, FGF acidic, FGF basic, HB-EGF, HGF, IGF1, IGF2, KGF, TGF beta 1, PD-ECGF, PDGF AA, PDGF AB, PDGF BB, PIGF, VEGF, VEGFC, VEGF R2 and VEGF R3. Mouse: GM-CSF, IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A, TNF alpha, VEGFD and VEGF R3. Rat: IFN gamma, IL1 beta, IL4, IL6, IL10 and TNF alpha. |
| Target Name | VEGFD |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.3 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 50 µl |
| Precision | Intra-Assay CV: 2.1% Inter-Assay CV: 3.2% |
| Alternate Names | Vascular endothelial growth factor D; VEGFD; c-Fos-induced growth factor; FIGF; VEGF-D |
Application Instructions
| Assay Time | ~ 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43915 Human Vascular endothelial growth factor D |
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| Gene Symbol | FIGF |
| Gene Full Name | c-fos induced growth factor (vascular endothelial growth factor D) |
| Background | The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C. Read-through transcription has been observed between this locus and the upstream PIR (GeneID 8544) locus. [provided by RefSeq, Feb 2011] |
| Function | Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82584 Human VEGFD ELISA Kit standard curve image
ARG82584 Human VEGFD ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82584 Human VEGFD ELISA Kit User manual |
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