ARG82258
Human VEGFB ELISA Kit
Human VEGFB ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82258 Human VEGFB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human VEGFB in serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | The following recombinant Human proteins were tested and exhibited no cross-reactivity or interference: ApoAI, BMP1, BMP2, BMP3, BMP4, BMP7, CRP, HGF, HSP27, IL1 alpha, IL1 RI, IL1 beta, IL1RA, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL15, IL17C, IL21, IL23, IFN gamma, MMP2, sIL2R, sIL6R, PDGF, PLA2G7, prolactin, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TLR3, TNF alpha, TNF RI, TNF RII, VEGFA. |
| Target Name | VEGFB |
| Conjugation | HRP |
| Conjugation Note | Read at 450 nm. |
| Sensitivity | 16 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 31 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6% Inter-Assay CV: 9% |
| Alternate Names | VEGF-related factor; VEGFL; VEGF-B; VRF; Vascular endothelial growth factor B |
Application Instructions
| Assay Time | ~ 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P49765 Human Vascular endothelial growth factor B |
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| Gene Symbol | VEGFB |
| Gene Full Name | vascular endothelial growth factor B |
| Background | This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Sep 2011] |
| Function | Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. [UniProt] |
| Cellular Localization | Secreted. Note=Secreted but remains associated to cells or to the extracellular matrix unless released by heparin. [UniProt] |
| Highlight | Related products: VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | VEGF-B186 is O-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82258 Human VEGFB ELISA Kit standard curve image
ARG82258 Human VEGFB ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82258 Human VEGFB ELISA Kit User manual |
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