ARG82915
Human Urokinase / uPA ELISA Kit
Human Urokinase / uPA ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82915 Human Urokinase / uPA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Urokinase / uPA in serum, plasma (heparin, EDTA), cell lysates and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Urokinase / uPA |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 10 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), cell lysates and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 μl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | PLAU; Plasminogen Activator, Urokinase; UPA; URK; Urokinase-Type Plasminogen Activator; U-Plasminogen Activator; EC 3.4.21.73; Plasminogen Activator, Urinary; EC 3.4.21; BDPLT5; U-PA; ATF; QPD |
Application Instructions
| Assay Time | 4 hours |
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Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P00749 Human Urokinase-type plasminogen activator |
|---|---|
| Gene Symbol | PLAU |
| Gene Full Name | Plasminogen Activator, Urokinase |
| Background | This gene encodes a secreted serine protease that converts plasminogen to plasmin. The encoded preproprotein is proteolytically processed to generate A and B polypeptide chains. These chains associate via a single disulfide bond to form the catalytically inactive high molecular weight urokinase-type plasminogen activator (HMW-uPA). HMW-uPA can be further processed into the catalytically active low molecular weight urokinase-type plasminogen activator (LMW-uPA). This low molecular weight form does not bind to the urokinase-type plasminogen activator receptor. Mutations in this gene may be associated with Quebec platelet disorder and late-onset Alzheimer's disease. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016] |
| Function | Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. [Uniprot] |
| Cellular Localization | Secreted. [Uniprot] |
| PTM | Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen. [Uniprot] |
Images (1) Click the Picture to Zoom In
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ARG82915 Human Urokinase / uPA ELISA Kit standard curve image
ARG82915 Human Urokinase / uPA ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82915 Human Urokinase / uPA ELISA Kit User manual |
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