ARG81599
Human Trypsin (pan) ELISA Kit
Human Trypsin (pan) ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81599-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81599-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81599-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81599-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81599-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81599-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81599-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81599-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81599-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81599-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81599-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81599 Human Trypsin (pan) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Trypsin (pan) in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Trypsin (pan) |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.4% Inter-Assay CV: 8.6% |
| Alternate Names | Trypsin III; T9; Serine protease 3; TRY4; TRY3; EC 3.4.21.4; Serine protease 4; Trypsin-3; PRSS4; Mesotrypsinogen; Trypsin IV; Brain trypsinogen; MTG |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRSS3 |
| Gene Full Name | protease, serine, 3 |
| Background | This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2010] |
| Function | Digestive protease specialized for the degradation of trypsin inhibitors. In the ileum, may be involved in defensin processing, including DEFA5. [UniProt] |
| Highlight | Related products: Trypsin antibodies; Trypsin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81599 Human Trypsin (pan) ELISA Kit standard curve image
ARG81599 Human Trypsin (pan) ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81599 Human Trypsin (pan) ELISA Kit User's manual |
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