ARG81469
Human Tissue Factor Pathway Inhibitor ELISA Kit
Human Tissue Factor Pathway Inhibitor ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81469-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81469-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81469-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81469-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81469-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81469-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81469-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81469-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81469-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81469-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81469-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81469 Human Tissue Factor Pathway Inhibitor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Tissue Factor Pathway Inhibitor in serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Tissue Factor Pathway Inhibitor |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2% Inter-Assay CV: 7.0% |
| Alternate Names | TFI; Lipoprotein-associated coagulation inhibitor; LACI; EPI; TFPI1; Tissue factor pathway inhibitor; TFPI; Extrinsic pathway inhibitor |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TFPI |
| Gene Full Name | tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) |
| Background | This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq, Jul 2008] |
| Function | Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. [UniProt] |
| Highlight | Related products: Tissue Factor antibodies; Tissue Factor ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | O-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81469 Human Tissue Factor Pathway Inhibitor ELISA Kit standard curve image
ARG81469 Human Tissue Factor Pathway Inhibitor ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81469 Human Tissue Factor Pathway Inhibitor ELISA Kit User manual |
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