ARG80944
Human Thioredoxin-1/Trx-1 ELISA Kit
Human Thioredoxin-1/Trx-1 ELISA Kit for ELISA and Human
Metabolism kit; Signaling Transduction kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80944-001 | Antibody-coated microplate | 96 well | 4°C |
| ARG80944-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80944-003 | Sample Diluent | 20 ml | 4°C |
| ARG80944-004 | Biotinylated Trx1 antibody (30X) | 1 vial | 4°C |
| ARG80944-005 | Antibody Diluent | 16 ml | 4°C |
| ARG80944-006 | Streptavidin-HRP | 1 vial | 4°C |
| ARG80944-007 | Streptavidin-HRP Diluent | 16 ml | 4°C |
| ARG80944-008 | TMB Substrate | 12 ml | 4°C (Protect from light) |
| ARG80944-009 | 20X Wash buffer | 50 ml | 4°C |
| ARG80944-010 | Plate sealer | 4 strips | Room temperature |
| ARG80944-011 | STOP solution | 12 ml | 4°C |
Overview
| Product Description | ARG80944 Human Thioredoxin-1/Trx-1 ELISA Kit is a Enzyme Immunoassay kit for the quantification of Human Thioredoxin-1/Trx-1 in Serum, Plasma and Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | TRX1 / Thioredoxin 1 |
| Sensitivity | 62.5 pg/ml |
| Sample Type | Serum, Plasma and Cell culture supernatants |
| Standard Range | 125 - 8000 pg/ml |
| Sample Volume | 100 μl |
| Alternate Names | ATL-derived factor; TRX1; SASP; Trx; ADF; TRX; Surface-associated sulphydryl protein; TRDX; Thioredoxin |
Application Instructions
| Assay Time | ~200 min |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TXN |
| Gene Full Name | thioredoxin |
| Background | The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Function | Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55). [UniProt] |
| Highlight | Related products: TRX1 antibodies; TRX1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Metabolism kit; Signaling Transduction kit |
| PTM | In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins. In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation. |
Images (1) Click the Picture to Zoom In
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ARG80944 Human Thioredoxin-1/Trx-1 ELISA Kit standard curve image
ARG80944 Human Thioredoxin-1/Trx-1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80944 Human Thioredoxin-1/Trx-1 ELISA Kit User's manual |
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