ARG80854

Human Testosterone ELISA Kit

Human Testosterone ELISA Kit for ELISA and Human

Developmental Biology kit; Signaling Transduction kit

Overview

Product Description ARG80854 Human Testosterone ELISA Kit is an enzyme immunoassay kit for the quantification of Testosterone in serum and plasma (EDTA, heparin, citrate).
Tested Reactivity Hu
Tested Application ELISA
Target Name Testosterone
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.083 ng/ml
Sample Type Serum and plasma (EDTA, heparin, citrate).
Standard Range 0.2 - 16 ng/ml
Sample Volume 25 µl

Application Instructions

Assay Time 60, 15 min

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Testosterone
Background Testosterone (17β-hydroxy-4-androstene-3-one) is a C19 steroid with an unsaturated bond between C-4 and C-5, a ketone group in C-3 and a hydroxyl group in the β position at C-17.

This steroid hormone has a molecular weight of 288.47. Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes; in females ca. 50% of circulating testosterone is derived from peripheral conversion of androstenedione, ca. 25% from the ovary and ca. 25% from the adrenal glands.

Testosterone is responsible for the development of secondary male sex characteristics and its measurements are helpful in evaluating the hypogonadal states. In women, high levels of testosterone are generally found in hirsutism and virilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer. Low levels of testosterone can be found in patients with the following diseases: Hypopituitarism, Klinefelter's syndrome, Testicular feminization, Orchidectomy and Cryptorrchidism, enzymatic defects and some autoimmune diseases.
Highlight Related products:
Testosterone antibodies; Testosterone ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Developmental Biology kit; Signaling Transduction kit

Images (1) Click the Picture to Zoom In

  • ARG80854 Human Testosterone ELISA Kit example of standard curve image

    ARG80854 Human Testosterone ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG80854 Human Testosterone ELISA Kit User's manual download icon Download