ARG82370
Human Tamm Horsfall Glycoprotein ELISA Kit
Human Tamm Horsfall Glycoprotein ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82370-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82370-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82370-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82370-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82370-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82370-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82370-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82370-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82370-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82370-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82370-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82370 Human Tamm Horsfall Glycoprotein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Tamm Horsfall Glycoprotein in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Tamm Horsfall Glycoprotein |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2% Inter-Assay CV: 6.4% |
| Alternate Names | Uromodulin; HNFJ; MCKD2; THGP; HNFJ1; FJHN; THP; Tamm-Horsfall urinary glycoprotein; ADMCKD2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | UMOD |
| Gene Full Name | uromodulin |
| Background | The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| Function | Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure providing the water barrier permeability. May serve as a receptor for binding and endocytosis for cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelial. Uromodulin, secreted form: Secreted into urine after proteolytically cleaveage. Into the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, prevents urinary tract infection and modulates formation of supersaturated salts and their crystals. [UniProt] |
| Cellular Localization | Apical cell membrane; Lipid-anchor, GPI-anchor. Basolateral cell membrane; Lipid-anchor, GPI-anchor. Cell projection, cilium membrane. Note=Only a small fraction sorts to the basolateral pole of tubular epithelial cells compared to apical localization (PubMed:22776760). Secreted into urine after cleavage (PubMed:18375198, PubMed:26811476). Colocalizes with NPHP1 and KIF3A (PubMed:20172860). Uromodulin, secreted form: Secreted. Note=Detected in urine. [UniProt] |
| Highlight | Related products: Glycoprotein antibodies; Glycoprotein ELISA Kits; Glycoprotein Duos / Panels; Glycoprotein recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated (PubMed:19005207, PubMed:26673890, PubMed:26811476). N-glycan heterogeneity at Asn-232: Hex7HexNAc6 (major) and dHex1Hex7HexNAc6 (minor); at Asn-322: dHex1Hex6HexNAc5 (minor), dHex1Hex7HexNAc6 (major) and dHex1Hex8HexNAc7 (minor); at Asn-396: Hex6HexNAc5 (major), dHex1Hex6HexNAc5 (minor) and Hex7HexNAc6 (minor) (PubMed:22171320). Proteolytically cleaved at a conserved C-terminal proteolytic cleavage site to generate the secreted form found in urine (PubMed:18375198, PubMed:19005207). This cleavage is catalyzed by HPN (PubMed:26673890). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82370 Human Tamm Horsfall Glycoprotein ELISA Kit standard curve image
ARG82370 Human Tamm Horsfall Glycoprotein ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82370 Human Tamm Horsfall Glycoprotein ELISA Kit User manual |
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