ARG81773
Human TSP1 / Thrombospondin 1 ELISA Kit
Human TSP1 / Thrombospondin 1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81773-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81773-002 | Standard | 2 X 100 ng/vial | 4°C |
| ARG81773-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81773-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81773-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81773-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81773-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81773-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81773-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81773-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81773-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81773 Human TSP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TSP in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TSP1 / Thrombospondin 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.78 ng/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 1.56 - 100 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.7%; Inter-Assay CV: 7.8% |
| Alternate Names | TSP1; THBS; THBS-1; Thrombospondin-1; TSP-1; TSP |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | THBS1 |
| Gene Full Name | thrombospondin 1 |
| Background | The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008] |
| Function | Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity). [UniProt] |
| Highlight | Related products: TSP antibodies; TSP ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81773 Human TSP ELISA Kit standard curve image
ARG81773 Human TSP ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81773 Human TSP ELISA Kit User's manual |
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