ARG82732
Human TPP1 ELISA Kit
Human TPP1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82732-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82732-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82732-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82732-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82732-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82732-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82732-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82732-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82732-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82732-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82732-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82732 Human TPP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TPP1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | TPP1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.6% Inter-Assay CV: 6.0% |
| Alternate Names | EC 3.4.14.9; TPP-1; Tripeptidyl-peptidase I; GIG1; Lysosomal pepstatin-insensitive protease; SCAR7; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase 1; CLN2; LPIC; Cell growth-inhibiting gene 1 protein; TPP-I |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TPP1 |
| Gene Full Name | tripeptidyl peptidase I |
| Background | This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq, Jul 2008] |
| Function | Lysosomal serine protease with tripeptidyl-peptidase I activity (PubMed:11054422, PubMed:19038966, PubMed:19038967). May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases (PubMed:11054422, PubMed:19038966, PubMed:19038967). Requires substrates with an unsubstituted N-terminus (PubMed:19038966). [UniProt] |
| Cellular Localization | Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [UniProt] |
| Highlight | Related products: TPP1 antibodies; TPP1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82732 Human TPP1 ELISA Kit standard curve image
ARG82732 Human TPP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82732 Human TPP1 ELISA kit User manual |
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