ARG81453
Human TNFRSF18 ELISA Kit
Human TNFRSF18 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81453-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81453-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81453-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81453-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81453-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81453-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81453-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81453-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81453-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81453-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81453-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81453 Human TNFRSF18 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TNFRSF18 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TNFRSF18 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3% Inter-Assay CV: 7.0% |
| Alternate Names | Tumor necrosis factor receptor superfamily member 18; AITR; CD357; CD antigen CD357; Activation-inducible TNFR family receptor; GITR-D; GITR; Glucocorticoid-induced TNFR-related protein |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9Y5U5 Human Tumor necrosis factor receptor superfamily member 18 |
|---|---|
| Gene Symbol | TNFRSF18 |
| Gene Full Name | tumor necrosis factor receptor superfamily, member 18 |
| Background | This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Feb 2011] |
| Function | Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. [UniProt] |
| Highlight | Related products: TNFRSF antibodies; TNFRSF ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81453 Human TNFRSF18 ELISA Kit standard curve image
ARG81453 Human TNFRSF18 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81453 Human TNFRSF18 ELISA Kit User's manual |
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