ARG81476
Human TLR3 ELISA Kit
Human TLR3 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81476-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81476-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81476-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81476-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81476-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81476-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81476-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81476-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81476-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81476-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81476-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81476 Human TLR3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TLR3 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TLR3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.8% Inter-Assay CV: 7.5% |
| Alternate Names | Toll-like receptor 3; CD antigen CD283; CD283; IIAE2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TLR3 |
| Gene Full Name | toll-like receptor 3 |
| Background | The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008] |
| Function | Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response. [UniProt] |
| Highlight | Related products: TLR3 antibodies; TLR3 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. TLR3 signaling requires a proteolytic cleavage mediated by cathepsins CTSB and CTSH, the cleavage occurs between amino acids 252 and 346. The cleaved form of TLR3 is the predominant form found in endosomes. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81476 Human TLR3 ELISA Kit standard curve image
ARG81476 Human TLR3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81476 Human TLR3 ELISA Kit User's manual |
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