ARG81815
Human TLR1 ELISA Kit
Human TLR1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81815-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81815-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81815-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81815-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81815-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81815-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81815-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81815-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81815-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81815-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81815-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81815 Human TLR1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TLR1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TLR1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2%; Inter-Assay CV: 7.4% |
| Alternate Names | CD281; CD antigen CD281; Toll/interleukin-1 receptor-like protein; TIL; Toll-like receptor 1; rsc786; TIL. LPRS5 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TLR1 |
| Gene Full Name | toll-like receptor 1 |
| Background | The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene. [provided by RefSeq, Jul 2008] |
| Function | Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). [UniProt] |
| Highlight | Related products: TLR1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81815 Human TLR1 ELISA Kit standard curve image
ARG81815 Human TLR1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81815 Human TLR1 ELISA Kit User manual |
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