ARG82596
Human TIE1 ELISA Kit
Human TIE1 ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82596 Human TIE1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TIE1 in serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | This kit could assay both natural and recombinant Human TIE1. No significant cross-reactivity or interference was observed in the following samples: Human: IFN gamma, IL1 beta, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL17A, IL18, IL21, IL22, IL23, MCP1, TGF beta 1, TNF alpha and VEGF. Mouse: GM-CSF, IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A and TNF alpha. Rat: IFN gamma, IL1 beta, IL4, IL6, IL10 and TNF alpha. |
| Target Name | TIE1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.16 ng/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 0.31 - 20 ng/ml |
| Sample Volume | 20 µl |
| Precision | Intra-Assay CV: 3.6% Inter-Assay CV: 2.5% |
| Alternate Names | TIE; Tyrosine-protein kinase receptor Tie-1; JTK14; EC 2.7.10.1 |
Application Instructions
| Assay Time | ~ 2.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P35590 Human Tyrosine-protein kinase receptor Tie-1 |
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| Gene Symbol | TIE1 |
| Gene Full Name | tyrosine kinase with immunoglobulin-like and EGF-like domains 1 |
| Background | This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
| Function | Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Highlight | Related products: TIE1 antibodies; TIE1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82596 Human TIE1 ELISA Kit standard curve image
ARG82596 Human TIE1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82596 Human TIE1 ELISA Kit User manual |
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