ARG80136
Human TGF alpha ELISA Kit
Human TGF alpha ELISA Kit for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80136-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80136-002 | Standard (Lyophilized) | 2 X 0.5 ng/vial | 4°C |
| ARG80136-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80136-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80136-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80136-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80136-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80136-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80136-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80136-010 | STOP solution | 12ml | 4°C |
| ARG80136-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80136 Human TGF alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TGF alpha in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human Amphiregulin, HB-EGF, Betacellulin, HRG- alpha, EGF, SMDF, EGFR |
| Target Name | TGF alpha |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 4 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 7.8 - 500 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | ETGF; EGF-like TGF; TFGA; Protransforming growth factor alpha; TGF-alpha; TGF type 1 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P01135 Human Protransforming growth factor alpha |
|---|---|
| Gene Symbol | TGFA |
| Gene Full Name | transforming growth factor, alpha |
| Background | This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| Function | TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. [UniProt] |
| Highlight | Related products: TGF alpha antibodies; TGF alpha ELISA Kits; TGF alpha recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit |
Images (1) Click the Picture to Zoom In
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ARG80136 Human TGF alpha ELISA Kit standard curve image
ARG80136 Human TGF alpha ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80136 Human TGF alpha ELISA Kit User's manual |
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