ARG81532
Human TFF3 ELISA Kit
Human TFF3 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81532-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81532-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81532-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81532-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81532-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81532-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81532-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81532-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81532-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81532-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81532-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81532 Human TFF3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TFF3 in serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TFF3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.5% Inter-Assay CV: 6.8% |
| Alternate Names | P1B; TFI; ITF; Trefoil factor 3; Intestinal trefoil factor; hITF; hP1.B; Polypeptide P1.B |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TFF3 |
| Gene Full Name | trefoil factor 3 (intestinal) |
| Background | Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008] |
| Function | Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen). [UniProt] |
| Highlight | Related products: TFF3 antibodies; TFF3 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81532 Human TFF3 ELISA Kit standard curve image
ARG81532 Human TFF3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81532 Human TFF3 ELISA Kit User's manual |
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