ARG81561
Human Syndecan 4 ELISA Kit
Human Syndecan 4 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81561-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81561-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81561-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81561-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81561-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81561-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81561-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81561-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81561-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81561-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81561-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81561 Human Syndecan 4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Syndecan 4 in serum, plasma (EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Syndecan 4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0% Inter-Assay CV: 7.2% |
| Alternate Names | Amphiglycan; Ryudocan core protein; SYND4; Syndecan-4 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SDC4 |
| Gene Full Name | syndecan 4 |
| Background | The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008] |
| Function | Cell surface proteoglycan that bears heparan sulfate. [UniProt] |
| Highlight | Related products: Syndecan antibodies; Syndecan ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG81561 Human Syndecan 4 ELISA Kit standard curve image
ARG81561 Human Syndecan 4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81561 Human Syndecan 4 ELISA Kit User's manual |
 Download
|
