ARG81573

Human Stanniocalcin 1 ELISA Kit

Human Stanniocalcin 1 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81573-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81573-002 Standard 2 X 10 ng/vial 4°C
ARG81573-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81573-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81573-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81573-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81573-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81573-008 25X Wash buffer 20 ml 4°C
ARG81573-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81573-010 STOP solution 10 ml (Ready to use) 4°C
ARG81573-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81573 Human Stanniocalcin 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Stanniocalcin 1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Stanniocalcin 1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.1%
Inter-Assay CV: 5.8%
Alternate Names Stanniocalcin-1; STC-1; STC

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6781 Human STC1

Swiss-port # P52823 Human Stanniocalcin-1

Gene Symbol STC1
Gene Full Name stanniocalcin 1
Background This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The gene contains a 5' UTR rich in CAG trinucleotide repeats. The encoded protein contains 11 conserved cysteine residues and is phosphorylated by protein kinase C exclusively on its serine residues. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Overexpression of human stanniocalcin 1 in mice produces high serum phosphate levels, dwarfism, and increased metabolic rate. This gene has altered expression in hepatocellular, ovarian, and breast cancers. [provided by RefSeq, Jul 2008]
Function Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia. [UniProt]
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Images (1) Click the Picture to Zoom In

  • ARG81573 Human Stanniocalcin 1 ELISA Kit standard curve image

    ARG81573 Human Stanniocalcin 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81573 Human Stanniocalcin 1 ELISA Kit User's manual download icon Download