ARG82720
Human Serpin D1 / Heparin cofactor II ELISA Kit
Human Serpin D1 / Heparin cofactor II ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82720-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82720-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG82720-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82720-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82720-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82720-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82720-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82720-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82720-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82720-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82720-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82720 Human Serpin D1 / Heparin cofactor II ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Serpin D1 / Heparin cofactor II in serum, plasma (EDTA, citrate), cell culture supernatants and urine. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Serpin D1 / Heparin cofactor II |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.39 ng/ml |
| Sample Type | Serum, plasma (EDTA, citrate), cell culture supernatants and urine. |
| Standard Range | 0.78 - 50 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.7% Inter-Assay CV: 6.1% |
| Alternate Names | HC2; Protease inhibitor leuserpin-2; D22S673; HC-II; HCII; THPH10; Heparin cofactor 2; Heparin cofactor II; HCF2; LS2; HLS2; Serpin D1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SERPIND1 |
| Gene Full Name | serpin peptidase inhibitor, clade D (heparin cofactor), member 1 |
| Background | This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq, Jul 2015] |
| Function | Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils. [UniProt] |
| Highlight | Related products: Serpin antibodies; Serpin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated by FAM20C in the extracellular medium. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82720 Human Serpin D1 / Heparin cofactor II ELISA Kit standard curve image
ARG82720 Human Serpin D1 / Heparin cofactor II ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82720 Human Serpin D1 / Heparin cofactor II ELISA Kit User manual |
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