ARG81492

Human Sclerostin ELISA Kit

Human Sclerostin ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81492-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81492-002 Standard 2 X 10 ng/vial 4°C
ARG81492-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81492-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81492-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81492-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81492-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81492-008 25X Wash buffer 20 ml 4°C
ARG81492-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81492-010 STOP solution 10 ml (Ready to use) 4°C
ARG81492-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81492 Human Sclerostin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Sclerostin in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Sclerostin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.5%
Inter-Assay CV: 6.5%
Alternate Names CDD; SOST1; Sclerostin; VBCH

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 50964 Human SOST

Swiss-port # Q9BQB4 Human Sclerostin

Gene Symbol SOST
Gene Full Name sclerostin
Background Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease. [provided by RefSeq, Jul 2008]
Function Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. [UniProt]
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Images (1) Click the Picture to Zoom In

  • ARG81492 Human Sclerostin ELISA Kit standard curve image

    ARG81492 Human Sclerostin ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81492 Human Sclerostin ELISA Kit User's manual download icon Download