ARG81535
Human SPINK1 ELISA Kit
Human SPINK1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81535-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81535-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81535-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81535-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81535-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81535-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81535-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81535-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81535-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81535-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81535-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81535 Human SPINK1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SPINK1 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | SPINK1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.1% Inter-Assay CV: 7.6% |
| Alternate Names | TATI; Serine protease inhibitor Kazal-type 1; Pancreatic secretory trypsin inhibitor; TCP; Tumor-associated trypsin inhibitor; PCTT; PSTI; Spink3 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P00995 Human Serine protease inhibitor Kazal-type 1 |
|---|---|
| Gene Symbol | SPINK1 |
| Gene Full Name | serine peptidase inhibitor, Kazal type 1 |
| Background | The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. [provided by RefSeq, Oct 2008] |
| Function | This is a trypsin inhibitor, its physiological function is to prevent the trypsin-catalyzed premature activation of zymogens within the pancreas. [UniProt] |
| Highlight | Related products: SPINK1 antibodies; SPINK1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81535 Human SPINK1 ELISA Kit standard curve image
ARG81535 Human SPINK1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81535 Human SPINK1 ELISA Kit User manual |
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