ARG82482
Human SMPD1 / Acid Sphingomyelinase ELISA Kit
Human SMPD1 / Acid Sphingomyelinase ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82482-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82482-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82482-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82482-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82482-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82482-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82482-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82482-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82482-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82482-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82482-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82482 Human SMPD1 / Acid Sphingomyelinase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SMPD1 / Acid Sphingomyelinase in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | SMPD1 / Acid Sphingomyelinase |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 10 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.7% Inter-Assay CV: 6.5% |
| Alternate Names | aSMase; EC 3.1.4.12; NPD; ASMASE; Sphingomyelin phosphodiesterase; Acid sphingomyelinase; ASM |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SMPD1 |
| Gene Full Name | sphingomyelin phosphodiesterase 1, acid lysosomal |
| Background | The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010] |
| Function | Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. [UniProt] |
| Cellular Localization | Lysosome. Secreted. [UniProt] |
| Highlight | Related products: SMPD1 / Acid Sphingomyelinase antibodies; SMPD1 / Acid Sphingomyelinase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82482 Human SMPD1 / Acid Sphingomyelinase ELISA Kit standard curve image
ARG82482 Human SMPD1 / Acid Sphingomyelinase ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82482 Human SMPD1 / Acid Sphingomyelinase ELISA Kit User manual |
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