ARG81585
Human SFRP5 ELISA Kit
Human SFRP5 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81585-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81585-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81585-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81585-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81585-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81585-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81585-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81585-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81585-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81585-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81585-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81585 Human SFRP5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SFRP5 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | SFRP5 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.6% Inter-Assay CV: 6.7% |
| Alternate Names | Secreted apoptosis-related protein 3; Secreted frizzled-related protein 5; SARP3; Frizzled-related protein 1b; SARP-3; FRP-1b; sFRP-5 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q5T4F7 Human Secreted frizzled-related protein 5 |
|---|---|
| Gene Symbol | SFRP5 |
| Gene Full Name | secreted frizzled-related protein 5 |
| Background | Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq, Jul 2008] |
| Function | Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81585 Human SFRP5 ELISA Kit standard curve image
ARG81585 Human SFRP5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81585 Human SFRP5 ELISA Kit User's manual |
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