ARG70415
Human SCF recombinant protein (Active) (His-tagged, C-ter)
Human SCF recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human SCF recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | SCF |
| Species | Human |
| A.A. Sequence | Glu26 - Ala189 |
| Expression System | E. coli |
| Protein Full Name | Kit ligand |
| Activity | Active |
| Activity Note | Determined by its ability to induce TF-1 cells proliferation. The ED50 for this effect is <5 ng/mL. The specific activity of recombinant human SCF is > 5 x 10^5 IU/mg. |
| Alternate Names | KITLG; KIT Ligand; SCF; Mast Cell Growth Factor; Stem Cell Factor; DFNA69; Kitl; KL-1 FPH2; SLF 2; MGF; SF; Familial Progressive Hyperpigmentation 2; Steel Factor; C-Kit Ligand; Kit Ligand; SHEP7; CUA; FPHH; WS2F |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | KITLG |
|---|---|
| Gene Full Name | KIT Ligand |
| Background | This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. |
| Function | Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. |
| Cellular Localization | Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Secreted |
| PTM | Disulfide bond; Glycoprotein |
Images (1) Click the Picture to Zoom In
-
ARG70415 Human SCF recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70415 Human SCF recombinant protein (Active) (His-tagged, C-ter)
