ARG81516
Human S100A12 ELISA Kit
Human S100A12 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81516-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81516-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81516-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81516-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81516-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81516-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81516-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81516-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81516-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81516-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81516-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81516 Human S100A12 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human S100A12 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | S100A12 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3% Inter-Assay CV: 7.7% |
| Alternate Names | Calgranulin-C; S100 calcium-binding protein A12; p6; Neutrophil S100 protein; Calcium-binding protein in amniotic fluid 1; MRP-6; ENRAGE; Migration inhibitory factor-related protein 6; CAAF1; EN-RAGE; Protein S100-A12; CAGC; Extracellular newly identified RAGE-binding protein; CGRP; MRP6 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | S100A12 |
| Gene Full Name | S100 calcium binding protein A12 |
| Background | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity. [provided by RefSeq, Nov 2014] |
| Function | S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. [UniProt] |
| Highlight | Related products: S100A antibodies; S100A ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81516 Human S100A12 ELISA Kit standard curve image
ARG81516 Human S100A12 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81516 Human S100A12 ELISA Kit User's manual |
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