ARG82385
Human Robo1 / DUTT1 ELISA Kit
Human Robo1 / DUTT1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82385-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82385-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82385-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82385-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82385-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82385-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82385-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82385-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82385-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82385-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82385-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82385 Human Robo1 / DUTT1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Robo1 / DUTT1 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Robo1 / DUTT1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.9% Inter-Assay CV: 6.5% |
| Alternate Names | Deleted in U twenty twenty; H-Robo-1; DUTT1; Roundabout homolog 1; SAX3 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ROBO1 |
| Gene Full Name | roundabout guidance receptor 1 |
| Background | Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| Function | Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Note=Detected at growth cones in thalamus neurons. [UniProt] |
| Highlight | Related products: Robo1 antibodies; Robo1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Ubiquitinated. May be deubiquitinated by USP33. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82385 Human Robo1 / DUTT1 ELISA Kit standard curve image
ARG82385 Human Robo1 / DUTT1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82385 Human Robo1 / DUTT1 ELISA Kit User manual |
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