ARG82531
Human Renalase ELISA Kit
Human Renalase ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82531-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82531-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82531-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82531-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82531-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82531-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82531-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82531-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82531-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82531-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82531-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82531 Human Renalase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Renalase in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Renalase |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.3% Inter-Assay CV: 6.6% |
| Alternate Names | alpha-NAD; MAO-C; Monoamine oxidase-C; EC 1.6.3.5; RENALASE; P; C10orf59; Renalase |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RNLS |
| Gene Full Name | renalase, FAD-dependent amine oxidase |
| Background | Renalase is a flavin adenine dinucleotide-dependent amine oxidase that is secreted into the blood from the kidney (Xu et al., 2005 [PubMed 15841207]).[supplied by OMIM, Mar 2008] |
| Function | Catalyzes the oxidation of the less abundant alpha-NAD(P)H isoform to form beta-NAD(P)(+). The enzyme hormone is secreted by the kidney, and circulates in blood and modulates cardiac function and systemic blood pressure. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: Renalase antibodies; Renalase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82531 Human Renalase ELISA Kit standard curve image
ARG82531 Human Renalase ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82531 Human Renalase ELISA Kit User manual |
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