ARG81589
Human RSPO4 ELISA Kit
Human RSPO4 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81589-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81589-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81589-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81589-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81589-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81589-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81589-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81589-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81589-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81589-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81589-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81589 Human RSPO4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human RSPO4 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | RSPO4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.8% Inter-Assay CV: 6.6% |
| Alternate Names | CRISTIN4; Roof plate-specific spondin-4; hRspo4; R-spondin-4; C20orf182 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RSPO4 |
| Gene Full Name | R-spondin 4 |
| Background | This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009] |
| Function | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Tyr-112 may be phosphorylated; however as this position is probably extracellular, the vivo relevance is not proven. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81589 Human RSPO4 ELISA Kit standard curve image
ARG81589 Human RSPO4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81589 Human RSPO4 ELISA Kit User's manual |
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