ARG81597
Human RSPO1 ELISA Kit
Human RSPO1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81597-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81597-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81597-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81597-004 | Antibody conjugate concentrate (100X) | 1 vial (120 µl) | 4°C |
| ARG81597-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81597-006 | HRP-Streptavidin concentrate (100X) | 1 vial (120 µl) | 4°C |
| ARG81597-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81597-008 | 25X Wash buffer | 30 ml | 4°C |
| ARG81597-009 | TMB substrate | 12 ml (Ready to use) | 4°C (Protect from light) |
| ARG81597-010 | STOP solution | 12 ml (Ready to use) | 4°C |
| ARG81597-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81597 Human RSPO1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human RSPO1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | RSPO1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.1% Inter-Assay CV: 5.9% |
| Alternate Names | hRspo1; Roof plate-specific spondin-1; R-spondin-1; RSPO; CRISTIN3 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RSPO1 |
| Gene Full Name | R-spondin 1 |
| Background | This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| Function | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. [UniProt] |
| Highlight | Related products: RSPO1 antibodies; RSPO1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81597 Human RSPO1 ELISA Kit standard curve image
ARG81597 Human RSPO1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81597 Human RSPO1 ELISA Kit User's manual |
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