ARG82390
Human REG1 alpha ELISA Kit
Human REG1 alpha ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82390-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82390-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82390-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82390-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82390-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82390-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82390-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82390-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82390-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82390-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82390-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82390 Human REG1 alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human REG1 alpha in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | REG1 alpha |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.8% Inter-Assay CV: 6.3% |
| Alternate Names | Islet cells regeneration factor; Regenerating protein I alpha; Lithostathine-1-alpha; Islet of Langerhans regenerating protein; PSPS1; ICRF; REG-1-alpha; Regenerating islet-derived protein 1-alpha; P19; PSP; Pancreatic thread protein; PSPS; REG; Pancreatic stone protein; PTP |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | REG1A |
| Gene Full Name | regenerating islet-derived 1 alpha |
| Background | This gene is a type I subclass member of the Reg gene family. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. Reg family members REG1B, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication. [provided by RefSeq, Jul 2008] |
| Function | Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: REG1 alpha antibodies; REG1 alpha ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The composition of the O-linked carbohydrate on Thr-27 is complex and varied. In the crystallographic structure, the attached sugar appears to be N-acetylglucosamine, typical of an intracellular protein, rather than N-acetylgalactosamine. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82390 Human REG1 alpha ELISA Kit standard curve image
ARG82390 Human REG1 alpha ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82390 Human REG1 alpha ELISA Kit User manual |
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