ARG81754
Human RANK ELISA Kit
Human RANK ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81754-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81754-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81754-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81754-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81754-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81754-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81754-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81754-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81754-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81754-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81754-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81754 Human RANK ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human RANK in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | RANK |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2%; Inter-Assay CV: 6.5% |
| Alternate Names | ODFR; Osteoclast differentiation factor receptor; OFE; FEO; CD265; PDB2; CD antigen CD265; RANK; Receptor activator of NF-KB; OPTB7; Tumor necrosis factor receptor superfamily member 11A; TRANCER; LOH18CR1; OSTS |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9Y6Q6 Human Tumor necrosis factor receptor superfamily member 11A |
|---|---|
| Gene Symbol | TNFRSF11A |
| Gene Full Name | tumor necrosis factor receptor superfamily, member 11a, NFKB activator |
| Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Aug 2012] |
| Function | Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. [UniProt] |
| Highlight | Related products: RANK antibodies; RANK ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81754 Human RANK ELISA Kit standard curve image
ARG81754 Human RANK ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81754 Human RANK ELISA Kit User's manual |
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