ARG82748
Human QPCT ELISA Kit
Human QPCT ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82748-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82748-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82748-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82748-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82748-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82748-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82748-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82748-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82748-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82748-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82748-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82748 Human QPCT ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human QPCT in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | QPCT |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3% Inter-Assay CV: 6.3% |
| Alternate Names | EC 2.3.2.5; Glutaminyl cyclase; Glutamyl cyclase; EC; QC; Glutaminyl-peptide cyclotransferase; GCT; sQC; Glutaminyl-tRNA cyclotransferase |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q16769 Human Glutaminyl-peptide cyclotransferase |
|---|---|
| Gene Symbol | QPCT |
| Gene Full Name | glutaminyl-peptide cyclotransferase |
| Background | This gene encodes human pituitary glutaminyl cyclase, which is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. The amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase. [provided by RefSeq, Jul 2008] |
| Function | Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. Also catalyzes N-terminal pyroglutamate formation. In vitro, catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-beta-amyloid. May be involved in the N-terminal pyroglutamate formation of several amyloid-related plaque-forming peptides. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: QPCT antibodies; QPCT ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82748 Human QPCT ELISA Kit standard curve image
ARG82748 Human QPCT ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82748 Human QPCT ELISA kit User manual |
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