ARG81619
Human Properdin ELISA Kit
Human Properdin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81619-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81619-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81619-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81619-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81619-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81619-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81619-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81619-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81619-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81619-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81619-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81619 Human Properdin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Properdin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Properdin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.0% Inter-Assay CV: 5.4% |
| Alternate Names | PFC; PFD; BFD; PROPERDIN; Complement factor P; Properdin |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CFP |
| Gene Full Name | complement factor properdin |
| Background | This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Feb 2009] |
| Function | A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. [UniProt] |
| Highlight | Related products: Properdin antibodies; Properdin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81619 Human Properdin ELISA Kit standard curve image
ARG81619 Human Properdin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81619 Human Properdin ELISA Kit User's manual |
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