ARG82394
Human Plexin B3 ELISA Kit
Human Plexin B3 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82394-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82394-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82394-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82394-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82394-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82394-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82394-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82394-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82394-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82394-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82394-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82394 Human Plexin B3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Plexin B3 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Plexin B3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 46.8 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 93.7 - 6000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0% Inter-Assay CV: 6.5% |
| Alternate Names | PLEXB3; PLXN6; PLEXR; Plexin-B3 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PLXNB3 |
| Gene Full Name | plexin B3 |
| Background | The protein encoded by this gene is a member of the plexin family. It functions as a receptor for semaphorin 5A, and plays a role in axon guidance, invasive growth and cell migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
| Function | Receptor for SEMA5A that plays a role in axon guidance, invasive growth and cell migration. Stimulates neurite outgrowth and mediates Ca(2+)/Mg(2+)-dependent cell aggregation. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Note=Colocalizes with RIT2/RIN at the plasma membrane. [UniProt] |
| Highlight | Related products: Plexin antibodies; Plexin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82394 Human Plexin B3 ELISA Kit standard curve image
ARG82394 Human Plexin B3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82394 Human Plexin B3 ELISA Kit User manual |
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