ARG83434
Human Plasminogen ELISA Kit
Human Plasminogen ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83434 Human Plasminogen ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Plasminogen in Serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | This ELISA kit is specific to Angiostatin K1-3. |
| Target Name | Plasminogen |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 10 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | EC 3.4.21.7; Plasminogen |
Application Instructions
| Assay Time | 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 4°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PLG |
| Gene Full Name | plasminogen |
| Background | The protein encoded by this gene is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasmin dissolves fibrin in blood clots and is an important protease in many other cellular processes while angiostatin inhibits angiogenesis. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009] |
| Function | Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. [UniProt] |
| Cellular Localization | Secreted. Note=Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface. [UniProt] |
| PTM | Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen |
Images (1) Click the Picture to Zoom In
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ARG83434 Human Plasminogen ELISA Kit standard curve image
ARG83434 Human Plasminogen ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83434 Human Plasminogen ELISA Kit User manual |
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