ARG82428
Human Phospholipase A2 IIA ELISA Kit
Human Phospholipase A2 IIA ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82428-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82428-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82428-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82428-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82428-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82428-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82428-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82428-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82428-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82428-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82428-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82428 Human Phospholipase A2 IIA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Phospholipase A2 IIA in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Phospholipase A2 IIA |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3% Inter-Assay CV: 6.1% |
| Alternate Names | PLA2B; GIIC sPLA2; EC 3.1.1.4; Non-pancreatic secretory phospholipase A2; PLA2; MOM1; PLA2L; PLAS1; PLA2S; NPS-PLA2; Group IIA phospholipase A2; sPLA2; Phospholipase A2, membrane associated; Phosphatidylcholine 2-acylhydrolase 2A |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P14555 Human Phospholipase A2, membrane associated |
|---|---|
| Gene Symbol | PLA2G2A |
| Gene Full Name | phospholipase A2, group IIA (platelets, synovial fluid) |
| Background | The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009] |
| Function | Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. [UniProt] |
| Cellular Localization | Cell membrane; Peripheral membrane protein. Secreted. [UniProt] |
| Highlight | Related products: Phospholipase antibodies; Phospholipase ELISA Kits; Phospholipase Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82428 Human Phospholipase A2 IIA ELISA Kit standard curve image
ARG82428 Human Phospholipase A2 IIA ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82428 Human Phospholipase A2 IIA ELISA Kit User maunal |
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