ARG82038
Human Peroxiredoxin 2 ELISA Kit
Human Peroxiredoxin 2 ELISA Kit for ELISA and Human
Publication2
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82038-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82038-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG82038-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82038-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82038-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82038-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82038-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82038-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82038-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82038-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82038-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82038 Human Peroxiredoxin 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Peroxiredoxin 2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Cross Reactivity: Human Recombinant Peroxiredoxin 1: 0.2% Human Recombinant Peroxiredoxin 3: 5.1% Human Recombinant Peroxiredoxin 4: 19.8% Human Recombinant Peroxiredoxin 5: 0.3% |
| Target Name | Peroxiredoxin 2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.6%; Inter-Assay CV: 6.6% |
| Alternate Names | EC 1.11.1.15; TPX1; TDPX1; HEL-S-2a; PTX1; Thiol-specific antioxidant protein; PRX2; TSA; Peroxiredoxin-2; PRP; Thioredoxin-dependent peroxide reductase 1; NKEF-B; NKEFB; Natural killer cell-enhancing factor B; Thioredoxin peroxidase 1; PRXII |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRDX2 |
| Gene Full Name | peroxiredoxin 2 |
| Background | This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8(+) T-cells. The crystal structure of this protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making this gene a therapeutic target for patients with hemolytic anemia. This protein may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13. [provided by RefSeq, Mar 2013] |
| Function | Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Highlight | Related products: Peroxiredoxin antibodies; Peroxiredoxin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82038 Human Peroxiredoxin 2 ELISA Kit standard curve image
ARG82038 Human Peroxiredoxin 2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
Executive dysfunction in euthymic bipolar disorder patients and its association with plasma biomarkers
ELISA / Human / Plasma
Identification of potential plasma protein biomarkers for bipolar II disorder: a preliminary/exploratory study.
ELISA / Human / Plasma
| Title | Download Link |
|---|---|
| ARG82038 Human Peroxiredoxin 2 ELISA Kit User manual |
 Download
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