ARG81496
Human Periostin ELISA Kit
Human Periostin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81496-001 | Antibody-Coated Microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81496-002 | Standard (Lyophilized) | 2 X 10 ng/vial | 4°C |
| ARG81496-003 | Standard/Sample Diluent | 30 ml (Ready to use) | 4°C |
| ARG81496-004 | Antibody Conjugate Concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81496-005 | Antibody Diluent Buffer | 12 ml (Ready to use) | 4°C |
| ARG81496-006 | HRP-Streptavidin Concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81496-007 | HRP-Streptavidin Diluent Buffer | 12 ml (Ready to use) | 4°C |
| ARG81496-008 | 25X Wash Buffer | 20 ml | 4°C |
| ARG81496-009 | TMB Substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81496-010 | STOP Solution | 10 ml (Ready to use) | 4°C |
| ARG81496-011 | Plate Sealers | 4 strips | Room temperature |
Overview
| Product Description | ARG81496 Human Periostin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Periostin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Periostin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3% Inter-Assay CV: 6.2% |
| Alternate Names | OSF2; PN; Osteoblast-specific factor 2; Periostin; OSF-2; PDLPOSTN |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | POSTN |
| Gene Full Name | periostin, osteoblast specific factor |
| Background | This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] |
| Function | Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity). Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. [UniProt] |
| Highlight | Related products: Periostin antibodies; Periostin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81496 Human Periostin ELISA Kit standard curve image
ARG81496 Human Periostin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81496 Human Periostin ELISA Kit User's manual |
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