ARG81429
Human PROK1 / EG-VEGF ELISA Kit
Human PROK1 / EG-VEGF ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81429-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81429-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81429-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81429-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81429-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81429-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81429-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81429-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81429-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81429-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81429-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81429 Human PROK1 / EG-VEGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PROK1 / EG-VEGF in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | PROK1 / EG-VEGF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2% Inter-Assay CV: 7.2% |
| Alternate Names | PK1; Endocrine-gland-derived vascular endothelial growth factor; Prokineticin-1; PRK1; EG-VEGF; Mambakine; EGVEGF |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PROK1 |
| Gene Full Name | prokineticin 1 |
| Background | The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011] |
| Function | Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. [UniProt] |
| Highlight | Related products: PROK1 antibodies; PROK1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81429 Human PROK1 / EG-VEGF ELISA Kit standard curve image
ARG81429 Human PROK1 / EG-VEGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81429 Human PROK1 / EG-VEGF ELISA Kit User's manual |
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