ARG82717
Human PODXL2 / Endoglycan ELISA Kit
Human PODXL2 / Endoglycan ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82717-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82717-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82717-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82717-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82717-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82717-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82717-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82717-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82717-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82717-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82717-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82717 Human PODXL2 / Endoglycan ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PODXL2 / Endoglycan in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | PODXL2 / Endoglycan |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2% Inter-Assay CV: 6.0% |
| Alternate Names | Endoglycan; EG; PODLX2; Podocalyxin-like protein 2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PODXL2 |
| Gene Full Name | podocalyxin-like 2 |
| Background | This gene is a member of the CD34 family of cell surface transmembrane proteins, which are characterized by an N-terminal extracellular mucin domain, globular and stalk domains, a single pass transmembrane region, and a charged cytoplasmic tail. The encoded protein is a ligand for vascular selectins. [provided by RefSeq, Oct 2012] |
| Function | Acts as a ligand for vascular selectins. Mediates rapid rolling of leukocytes over vascular surfaces through high affinity divalent cation-dependent interactions with E-, P- and L-selectins. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Glycosylated; contains chondroitin sulfate. Displays sialylated O-linked oligosaccharides. Sulfation is necessary for interaction with SELL. Sialylated O-linked oligosaccharides are necessary for interaction with SELL, SELE and SELP. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82717 Human PODXL2 / Endoglycan ELISA Kit standard curve image
ARG82717 Human PODXL2 / Endoglycan ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82717 Human PODXL2 / Endoglycan ELISA kit User manual |
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