ARG83344
Human PNP / Purine nucleoside phosphorylase ELISA Kit
Human PNP / Purine nucleoside phosphorylase ELISA Kit for ELISA and Human
Neuroscience antibody
Overview
| Product Description | ARG83344 Human PNP / Purine nucleoside phosphorylase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PNP / Purine nucleoside phosphorylase in serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | PNP / Purine nucleoside phosphorylase |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15 pg/ml |
| Detection Range | 31.2 pg/ml - 2000 pg/ml |
| Sample Type | Serum, Plasma and Cell culture supernatants. |
| Alternate Names | EC 2.4.2.1; Purine nucleoside phosphorylase; Inosine-guanosine phosphorylase; PNP; PRO1837; Inosine phosphorylase; PUNP; NP |
Application Instructions
| Assay Time | ~ 5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | PNP |
| Gene Full Name | purine nucleoside phosphorylase |
| Background | This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008] |
| Function | The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. [UniProt] |
| Cellular Localization | Cytoplasm, cytoskeleton. Cytoplasm |
| Research Area | Neuroscience antibody |
Images (1) Click the Picture to Zoom In
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ARG83344 Human PNP / Purine nucleoside phosphorylase ELISA Kit standard curve image
ARG83344 Human PNP / Purine nucleoside phosphorylase ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83344 Human PNP / Purine nucleoside phosphorylase ELISA Kit User manual |
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